The varied influences of cell adhesion and spreading on gene transfection of mesenchymal stem cells on a micropatterned substrate

Transmembrane transport of exogenous genes is widely investigated because high demand for gene therapy. Both carriers and cellular conditions can affect transfection efficiency. Although cell morphology has been reported to functions, the influence adhesion area spreading on remains unclear it difficult separate individual these areas during normal culture. In this study, micropatterns were prepared separately control human bone marrow-derived mesenchymal stem cells (hMSCs). Transfection efficiency green fluorescent protein hMSCs cultured was compared. Cells with a larger showed higher efficiency, while hardly affected Cell had dominant transfection. Microparticle uptake BrdU staining that capacity DNA synthesis activity increased increase in area, but not by area. The different correlated their capacity, activity, focal formation, cytoskeletal mechanics, mechanotransduction signal activation. results suggest transfection; finding should provide useful information manipulation functions therapy, modification, reprogramming. are important morphological factors interaction biomaterial surfaces or interfaces. However, predominant factor affects such as unclear. present special used precisely independently. Mesenchymal transfected compare transfection, did could be explained through formation FAs, YAP/TAZ nuclear localization. new insights correlation between designing functional biomaterials.